Medical and Dental Consultantsí Association of Nigeria
Home - About us - Editorial board - Search - Ahead of print - Current issue - Archives - Submit article - Instructions - Subscribe - Advertise - Contacts - Login 
  Users Online: 236   Home Print this page Email this page Small font sizeDefault font sizeIncrease font size
 
ORIGINAL ARTICLE
Year : 2010  |  Volume : 13  |  Issue : 3  |  Page : 284-287

Quantification of human immunodeficiency Virus -1 Viral load using nucleic acid sequence-based amplification (NASBA) in North Central Nigeria


Virology Research Laboratory, Innovative Biotech-Abuja/Keffi, Nigeria

Correspondence Address:
J C Forbi
Virology Research Laboratory, Innovative Biotech-Abuja/Keffi
Nigeria
Login to access the Email id

Source of Support: None, Conflict of Interest: None


PMID: 20857786

Rights and PermissionsRights and Permissions

Background: Viral load (VL) quantification is considered an integral part of the standard care in human immunodeficiency virus (HIV) infected individuals but in Nigeria as in most of sub-Saharan Africa, this has not reached the majority of patients. Methods: We report the first field application of the NucliSens EasyQ HIV-1 platform for the real time quantification of HIV-1 VL combining NASBA amplification and real time detection with molecular beacons among HIV-1 infected individuals in north central Nigeria where the predominant HIV-1 subtypes are CRF02_AG and G. CD4 + counts were enumerated using a fluorescence-activated cell sorter system. Results: Of one hundred and forty nine (n=149) plasma sample from patients with mean age of 32 years and made up of 77 males and 72 females, fifty {n = 50 (37.9%); 28 males and 22 females}had VLs below the lower detection limit (LDL=25 IU/ml) set by the assay while eighty- two {n = 82 (62.1%); 39 males and 43 females}had VL levels above the LDL. Furthermore, 13 of 82 (15.9%) patients with viral loads above the LDL had VLs between 26-1000 IU/ml while 69 (84.1%) had VLs of 1001-2400000 IU/ml. 17 (11.4%) of the samples could not be analyzed due to poor viral amplification. Among individuals with both CD4 + and VL results (n=56), those with CD4 + of 1-418 cell/μl presented with higher VL usually above 45,000 IU/ml when compared with those with CD4 + of over 500 cell/μl. Conclusion: Our findings highlight the pattern, usefulness and feasibility of VL quantification by NucliSens EasyQ in monitoring HIV-1 patients in Nigeria.


[PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed869    
    Printed44    
    Emailed0    
    PDF Downloaded154    
    Comments [Add]    

Recommend this journal