|Year : 2015 | Volume
| Issue : 2 | Page : 213-220
Effects of erbium-and chromium-doped yttrium scandium gallium garnet and diode lasers on the surfaces of restorative dental materials: A scanning electron microscope study
M Hatipoglu1, C Barutcigil2
1 Department of Periodontology, Faculty of Dentistry, Akdeniz University, Antalya, Turkey
2 Department of Restorative Dentistry, Faculty of Dentistry, Akdeniz University, Antalya, Turkey
|Date of Acceptance||21-Aug-2014|
|Date of Web Publication||10-Feb-2015|
Department of Periodontology, Faculty of Dentistry, Akdeniz University, 07058 Antalya
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Aim: The aim of this study is to evaluate the potential effects of laser irradiation, which is commonly performed in periodontal surgery, on the surfaces of restorative materials.
Materials and Methods: Five different restorative dental materials were used in this study, as follows: (1) Resin composite, (2) polyacid-modified resin composite (compomer), (3) conventional glass ionomer cement (GIC), (4) resin-modified glass ionomer cement (RMGIC), and (5) amalgam. Four cylindrical samples (8 mm diameter, 2 mm height) were prepared for each restorative material. In addition, four freshly extracted, sound human incisors teeth were selected. Two different laser systems commonly used in periodontal surgery were examined in this study: A 810 nm diode laser at a setting of 1 W with continuous-phase laser irradiation for 10 s, and an erbium-and chromium-doped yttrium scandium gallium garnet (Er, Cr: YSGG) laser at settings of 2.5 W, 3.25 W, and 4 W with 25 Hz laser irradiation for 10 s. Scanning electron microscopy (SEM) analysis was performed to evaluate the morphology and surface deformation of the restorative materials and tooth surfaces.
Results: According to the SEM images, the Er, Cr: YSGG laser causes irradiation markings that appear as demineralized surfaces on tooth samples. The Er, Cr: YSGG laser also caused deep defects on composite, compomer, and RMGIC surfaces because of its high power, and the ablation was deeper for these samples. High-magnification SEM images of GIC samples showed the melting and combustion effects of the Er, Cr: YSGG laser, which increased as the laser power was increased. In amalgam samples, neither laser left significant harmful effects at the lowest power setting. The diode laser did cause irradiation markings, but they were insignificant compared with those left by the Er, Cr: YSGG laser on the surfaces of the different materials and teeth.
Conclusion: Within the limitations of this study, it can be concluded that Er, Cr: YSGG laser irradiation could cause distortions of the surfaces of restorative materials. Diode lasers can be preferred for periodontal surgery.
Keywords: Erbium chromium-doped yttrium scandium gallium garnet, diode laser, restorative dental materials, scanning electron microscope
|How to cite this article:|
Hatipoglu M, Barutcigil C. Effects of erbium-and chromium-doped yttrium scandium gallium garnet and diode lasers on the surfaces of restorative dental materials: A scanning electron microscope study. Niger J Clin Pract 2015;18:213-20
|How to cite this URL:|
Hatipoglu M, Barutcigil C. Effects of erbium-and chromium-doped yttrium scandium gallium garnet and diode lasers on the surfaces of restorative dental materials: A scanning electron microscope study. Niger J Clin Pract [serial online] 2015 [cited 2020 May 28];18:213-20. Available from: http://www.njcponline.com/text.asp?2015/18/2/213/151044
| Introduction|| |
With their technological advancement, dental lasers are more frequently used as an alternative to traditional surgery for various esthetic and functional periodontal surgical procedures such as gingivectomy, gingivoplasty, surgical crown lengthening, vestibuloplasty, frenectomy, removal of soft tissue pathologies, subgingival curettage, periodontal pocket disinfection, and reduction of gingival hypertrophy, as well as scaling and root planning.  With the use of lasers, postoperative pain is substantially reduced, minimal damage occurs in and around the target organ, minimal bleeding occurs during surgery, the surgical area is cleaner, and the risk of bacterial contamination is reduced. ,, Despite such advantages, soft tissue lasers do affect the surrounding tissues and might cause crater-like defects on dental hard tissues during periodontal surgery. ,
Many patients in need of periodontal surgery may have direct or indirect restorations close to the area that would undergo surgical intervention, and the effects of the lasers used in periodontal surgery on these restorations have not been sufficiently investigated. Unintentional laser contact may cause various degrees of deterioration on the surfaces of these restorations, potentially leading to bacterial adhesion or esthetic impairment. ,, Furthermore, the magnitude of damage in the adjacent restorations might be severe enough to require the replacement of the restoration. The limited studies available in the literature on this subject have mostly investigated the effects of soft tissue lasers such as neodymium-doped yttrium aluminum garnet (Nd: YAG) and carbon dioxide (CO 2 ) lasers on indirect restorative materials, , and the effects of erbium-and chromium-doped yttrium scandium gallium garnet (Er, Cr: YSGG) lasers, which can also be used in soft tissue surgery, have not been investigated. Er, Cr: YSGG has a wavelength of 2780 nm and is well-absorbed by water and to a lesser extent, hydroxyapatite. , The water content of dental soft and hard tissues thus absorbs the energy produced by the Er, Cr: YSGG laser, which leads to the ablation of the target tissue.  Since the water content of soft tissues is higher than that of dental hard tissues, Er, Cr: YSGG lasers are used at lower levels of power for soft tissue procedures.
It is known that resin composites, compomers, glass ionomers, and sometimes amalgam are most frequently preferred for the restoration of the regions that are mostly exposed to periodontal laser surgery, such as root surfaces. Therefore, investigation of the effects of lasers on these restorative materials is important for clinicians to evaluate the risks of laser exposure during surgical procedures.  This issue might substantially affect the decisions of dentists that frequently use lasers in various soft tissue procedures, which are gradually becoming widespread. Given the historically strong interest in damage to dental hard tissues, surprisingly little attention has been directed toward the consequences of accidental laser irradiation of dental restorations.
The aim of this in-vitro study is to assess the potential effects of Er, Cr: YSGG and diode lasers, which are frequently used in periodontal surgery at various levels of power, on the surfaces of direct restorative materials by means of a scanning electron microscope (SEM).
| Materials and Methods|| |
Five different restorative dental materials were used in this study, as follows: (1) resin composite, (2) polyacid-modified resin composite (compomer), (3) conventional glass ionomer cement (GIC), (4) resin-modified glass ionomer cement (RMGIC), and (5) amalgam. The materials and their compositions are listed in [Table 1]. Four cylindrical samples (8 mm diameter, 2 mm height) were prepared for each restorative material using a Teflon mold on glass slabs, as per the manufacturers' directions. Photopolymerizable materials were set using a light-curing unit (3M ESPE Elipar S10, St. Paul, MN, USA), and the surfaces of the samples were polished with aluminum-oxide-coated disks (Sof-Lex; 3M ESPE, St. Paul, MN, USA). Amalgam samples were allowed to set for a minimum of 24 h before polishing. The samples were polished with a polishing kit (Kerr Identoflex Amalgam Polishers, Bioggio, Switzerland) after setting. In addition, four freshly extracted, sound human incisor teeth were selected. All calculus deposits and soft tissue were removed with a hand scaler. The teeth were sectioned vertically and stored in distilled water at 37°C. The lasers were irradiated to approximately 1 mm below the cement-enamel junction.
Two different laser systems commonly preferred in periodontal surgery were used in this study: An 810 nm diode laser (Epic, Biolase, Irvine, CA, USA) with a tip of 300 μm in diameter (e3 tip) and an Er, Cr: YSGG laser (Waterlase iPlus, Biolase, Irvine, CA, USA). The Er, Cr: YSGG laser system emits photons at a wavelength of 2.78 μm. The delivery system consisted of a fiber-optic tube terminating in a hand piece with a sapphire crystal tip bathed in an adjustable air-water spray. A G6 series tapered tip that was 600 μm in diameter and 6.0 mm in length was used.
Four different laser irradiation procedures were applied for each restorative material and tooth sample, as follows:
Scanning electron microscope investigation
- 2.5 W, 25 Hz Er, Cr: YSGG irradiation for 10 s, 40% water/20% air
- 3.25 W, 25 Hz Er, Cr: YSGG irradiation for 10 s, 40% water/20% air
- 4 W, 25 Hz Er, Cr: YSGG irradiation for 10 s, 40% water/20% air
- 1 W, continuous phase, 810 nm diode laser irradiation for 10 s.
Scanning electron microscopy analysis was performed to evaluate the changes in morphology and the surface deformation of the restorative materials and root surface. In the literature, SEM images are used extremely often for surface analysis. For this SEM evaluation, the samples were dried and sputter-coated with gold and palladium using a sputter-coating device (Polaron SC7620 Sputter Coater, VG Microtech, West Sussex, England). The SEM images were obtained using an electron microscope (Zeiss-Leo 1430 SEM, Angstrom Scientific Inc., NJ, USA) with magnifications of 100×, 500×, and 3000×.
| Results and Discussion|| |
Numerous studies have been conducted to investigate the effects of different types of lasers on dental surfaces. , It has been quite clearly demonstrated that CO 2 and Nd: YAG lasers have harmful effects on dental surfaces, and SEM images have revealed carbonized, cracked, and melted surfaces. ,, In addition, it has been showed that erbium-doped yttrium aluminum garnet (Er: YAG) lasers do not have such effects, but instead produce a surface similar to an acid-etched surface by removing the smear layer over the surface.  Hossain et al.  investigated the effects of Er, Cr: YSGG laser irradiation on dental hard tissues by SEM and reported that the laser leads to demineralization without causing crack formation and exposes dentin tubules. In the present study, the Er, Cr: YSGG laser did not cause cracks or fractures on dental surface, and the demineralization became deeper as the level of power was increased. The dentin surface became rough because of recrystallization, and there was also a lack of smear layer. On the dentin surface, the orifices of dentinal tubules were almost exposed, and intertubular dentin seemed to ablate more than peritubular dentin, leading to the protrusion of the dentinal tubules. The number and size of the dentin tubules increased with the power of the laser [Figure 1]. Mehl et al.  reported that such changes observed on dentin are associated with intensified laser effects. Although previous studies have demonstrated that diode lasers are safe, , in agreement with this study, it has been noted that they might have devastating effects on dental surfaces at high levels of power.  Kilinc et al.  showed that diode lasers only affected certain regions in one sample, and the surfaces of all other samples remained intact.
In the image of the resin composite after Er, Cr: YSGG laser irradiation [Figure 2], it is observed that the deformation, in general, bears combined traces of ablation, combustion, and melting. The deformation is deepened as the power of the laser is increased. The depth of the defect created by the laser pulse increased with the laser power. It has been shown that this relationship was logarithmic, with a very high correlation between laser power and ablation depth. Mazouri and Walsh  investigated the effect of CO 2 laser irradiation on esthetic restorative materials and stated, in line with this study that laser irradiation left burning and melting marks on composite surfaces. A previous study reported that the effects of lasers on composites change depending on the type of laser. The effect of CO 2 laser on a composite is irrecoverably devastating. The surfaces of the samples were carbonized, and the diameter of the damage was substantial. The effect of a Nd: YAG laser was also remarkable, but the magnitude of damage was not as high as that caused by the CO 2 laser. Although the effect of the diode laser was invisible, small defects and deformations have been detected at high magnification during SEM evaluation.  In the present study, the effect of diode laser irradiation on the surface of resin composites was very slight, as well.
|Figure 2 : Scanning electron microscopy images of resin composite samples|
Click here to view
The SEM images of the compomer samples are very similar to those of the composite samples [Figure 3]. In particular, the Er, Cr: YSGG laser caused deep defects on the compomer surfaces because of its high power, and the ablation was even deeper than that of the resin composite samples. The diode laser also caused deeper irradiation markings than those in the resin composite, but they were still insignificant compared with those caused by the Er, Cr: YSGG laser.
The Er, Cr: YSGG laser left shallow irradiation markings on conventional GIC. However, the high-magnification images show melting and combustion effects, which increased as the power was increased. This effect was especially apparent in the samples exposed to the 4 W laser irradiation. Superficial damage caused by the diode laser left an indistinct irradiation marking that could hardly be seen by the naked eye [Figure 4]. In the literature, there may be a deficiency in the studies investigating the effects of lasers on GIC. For this reason, the present study serves as a pilot study.
|Figure 4 : Scanning electron microscopy images of glass ionomer cement samples|
Click here to view
The SEM images of the surfaces of RMGIC showed deformations that look much more like those in the compomer and composite than those of the conventional GIC. This might have resulted from the resin matrix content of RMGIC. The Er, Cr: YSGG laser caused ablation defects on RMGIC similar to those in the materials with higher resin contents. This effect was more remarkable than that seen with other materials. The diode laser has a much smaller effect than the Er, Cr: YSGG laser. However, RMGIC was found to be less durable than the composite, compomer, and conventional glass ionomer against the diode laser [Figure 5]. Mazouri and Walsh  examined the irradiation markings left by a CO 2 laser on the surfaces of composite and compomer samples using a dissecting microscope. They reported that the effect of the CO 2 laser on composite samples was more remarkable than that on compomer materials. Nevertheless, they reported that materials containing resin with less or even no filler are more likely to experience ablation effects under laser irradiation.  Similarly, the present study found RMGIC, which does not include filler, to be the most affected material.
|Figure 5 : Scanning electron microscopy images of resin-modified glass ionomer cement samples|
Click here to view
For amalgam samples [Figure 6], the Er, Cr: YSGG laser had almost no harmful effects on the surface at the lowest power setting. The irradiation damage (markings) was deepened and widened as the laser power was increased. Previous studies have investigated the effects of different types of lasers on amalgam surfaces. , It was reported that an Nd: YAG laser caused harmful crater-like damage on amalgam surfaces that seemed to make replacement of the restoration necessary. However, CO 2 laser irradiation left a marking in the form of a fine line on the surface.  Similarly, the 810 nm diode laser left an indistinct irradiation marking that was hardly visible without magnification.  In the present study as well, the diode laser left an invisible irradiation marking on amalgam surface that could be detected at × 3000 × magnification. However, it has been reported that pits and defects, no matter how small, increase the risk of plaque retention and consequently corrosion of amalgam.  Mercury vapor, which would appear as a consequence of laser contact on the amalgam surface, should be kept in mind under such conditions, and appropriate suctioning should certainly be performed. 
Along with esthetic problems on existing restorations, fractures, cracks, bacterial adhesion, and plaque retention may also be caused by the lasers frequently used in surgical procedures for soft tissue, which may lead to secondary decay and periodontal problems. ,
Since shorter chair-side time is preferred in current dentistry practice, unplanned clinical complications that might lead to replacement of intact restorations are unacceptable and troublesome. The present study evaluated the effects of two different types of lasers widely used for soft tissue surgeries in clinical practice on traditional direct restorative materials and dental tissue. The aim was to facilitate the choice of the laser and mode specifically for the type of current restoration in the gingival surgery area.
Clinical conditions were mimicked as much as possible in the design of the experimental method of this present study. For example, the application of the laser to samples for 15 s by an expert operator was preferred rather than using a device that holds the end of a laser. The operator tried to apply the laser to the samples at a 45° angle along a straight line for 15 s. This was intended to imitate the contact that is likely to occur during a gingival surgical procedure on restorations.
Although the results of this study do not completely reflect the clinical in vivo conditions, the data and images have provided substantially important and detailed information. Nevertheless, information obtained in this study should be verified with long-term clinical studies.
Within the limitations of this in vitro study of the effects of lasers on restorative materials, it can be concluded that Er, Cr: YSGG lasers should not be used because of their irreversible effects on the surfaces, particularly at high power settings, unless absolutely necessary.
Since the 810 nm diode laser showed recoverable effects, it can be considered safe, even though it does affect most restorative materials. A routine polishing procedure is recommended for all restorative materials after procedures performed using diode lasers.
Further long-term clinical studies are necessary to validate the findings of this study.
| Acknowledgments|| |
This work was supported by The Scientific Research Projects Coordination Unit of Akdeniz University. Project Number: 2013.01.0151.003
| References|| |
Noori ZT, Fekrazad R, Eslami B, Etemadi A, Khosravi S, Mir M. Comparing the effects of root surface scaling with ultrasound instruments and Er, Cr: YSGG laser. Lasers Med Sci 2008;23:283-7.
Romanos G, Nentwig GH. Diode laser (980 nm) in oral and maxillofacial surgical procedures: Clinical observations based on clinical applications. J Clin Laser Med Surg 1999;17:193-7.
Parker S. Surgical laser use in implantology and endodontics. Br Dent J 2007;202:377-86.
Kato T, Kusakari H, Hoshino E. Bactericidal efficacy of carbon dioxide laser against bacteria-contaminated titanium implant and subsequent cellular adhesion to irradiated area. Lasers Surg Med 1998;23:299-309.
Goultschin J, Gazit D, Bichacho N, Bab I. Changes in teeth and gingiva of dogs following laser surgery: A block surface light microscope study. Lasers Surg Med 1988;8:402-8.
Pick RM, Pecaro BC, Silberman CJ. The laser gingivectomy. The use of the CO2 laser for the removal of phenytoin hyperplasia. J Periodontol 1985;56:492-6.
Cernavin I. A comparison of the effects of Nd: YAG and Ho: YAG laser irradiation on dentine and enamel. Aust Dent J 1995;40:79-84.
Noda M, Okuda Y, Tsuruki J, Minesaki Y, Takenouchi Y, Ban S. Surface damages of zirconia by Nd: YAG dental laser irradiation. Dent Mater J 2010;29:536-41.
Mazouri Z, Walsh LJ. Damage to dental composite restorations following exposure to CO2 laser radiation. J Clin Laser Med Surg 1995;13:73-6.
Eversole LR, Rizoiu I, Kimmel AI. Pulpal response to cavity preparation by an erbium, chromium: YSGG laser-powered hydrokinetic system. J Am Dent Assoc 1997;128:1099-106.
Hadley J, Young DA, Eversole LR, Gornbein JA. A laser-powered hydrokinetic system for caries removal and cavity preparation. J Am Dent Assoc 2000;131:777-85.
Jetter C. Soft-tissue management using an Er, Cr: YSGG laser during restorative procedures. Compend Contin Educ Dent 2008;29:46-9.
Tucker D, Cobb CM, Rapley JW, Killoy WJ. Morphologic changes following in vitro
CO2 laser treatment of calculus-ladened root surfaces. Lasers Surg Med 1996;18:150-6.
Israel M, Cobb CM, Rossmann JA, Spencer P. The effects of CO2, Nd: YAG and Er: YAG lasers with and without surface coolant on tooth root surfaces. An in vitro
study. J Clin Periodontol 1997;24:595-602.
Kilinc E, Rothrock J, Migliorati E, Drukteinis S, Roshkind DM, Bradley P. Potential surface alteration effects of laser-assisted periodontal surgery on existing dental restorations. Quintessence Int 2012;43:387-95.
Aoki A, Ando Y, Watanabe H, Ishikawa I. In vitro
studies on laser scaling of subgingival calculus with an erbium: YAG laser. J Periodontol 1994;65:1097-106.
Hossain M, Nakamura Y, Yamada Y, Suzuki N, Murakami Y, Matsumoto K. Analysis of surface roughness of enamel and dentin after Er, Cr: YSGG laser irradiation. J Clin Laser Med Surg 2001;19:297-303.
Mehl A, Kremers L, Salzmann K, Hickel R 3D volume-ablation rate and thermal side effects with the Er: YAG and Nd: YAG laser. Dent Mater 1997;13:246-51.
Kreisler M, Meyer C, Stender E, Daubländer M, Willershausen-Zönnchen B, d'Hoedt B. Effect of diode laser irradiation on the attachment rate of periodontal ligament cells: An in vitro
study. J Periodontol 2001;72:1312-7.
Schwarz F, Sculean A, Berakdar M, Szathmari L, Georg T, Becker J. In vivo
and in vitro
effects of an Er: YAG laser, a GaAlAs diode laser, and scaling and root planing on periodontally diseased root surfaces: A comparative histologic study. Lasers Surg Med 2003;32:359-66.
Cernavin I, Hogan SP. The effects of the Nd: YAG laser on amalgam dental restorative material. Aust Dent J 1999;44:98-102.
Shin SI, Min HK, Park BH, Kwon YH, Park JB, Herr Y, et al.
The effect of Er: YAG laser irradiation on the scanning electron microscopic structure and surface roughness of various implant surfaces: An in vitro
study. Lasers Med Sci 2011;26:767-76.
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6]
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