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ORIGINAL ARTICLE
Year : 2015  |  Volume : 18  |  Issue : 2  |  Page : 292-296

Effect of mineral trioxide aggregate and formocresol pulpotomy on vital primary teeth: A clinical and radiographic study


Department of Child Dental Health, Faculty of Dental Sciences, College of Medicine, University of Lagos, Lagos, Nigeria

Date of Acceptance16-Sep-2014
Date of Web Publication10-Feb-2015

Correspondence Address:
O O Olatosi
Department of Child Dental Health, Faculty of Dental Sciences, College of Medicine, University of Lagos, Lagos
Nigeria
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1119-3077.151071

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   Abstract 

Background: Pulpotomy is the common therapy for cariously exposed pulps in symptom-free primary molar teeth. Formocresol (FC) is considered the gold standard dressing agent for pulpotomy, but concerns have been raised over the years about its safety. Other alternative pulpotomy agents have been investigated and suggested.
Objective: The objective was to evaluate and compare the clinical and radiographic response of FC and white mineral trioxide aggregate (MTA) as pulpotomy materials on primary molars.
Materials and Methods: Fifty primary molars, with deep carious lesion that exposed a vital but asymptomatic pulp, in 37 children aged 4-7 years were treated with conventional pulpotomy procedure. The teeth were divided randomly into two groups. Group I (FC) and group II (MTA). The treated teeth were evaluated clinically and radiographically and were followed-up for 12 months.
Results:
At the end of the 12 months follow-up, the clinical success rates for FC and MTA were 81% and 100%, respectively. There was a statistically significant difference ( P = 0.04) between the clinical success rates of FC and MTA. While the radiographic success rates for FC and MTA were 81% and 96%, respectively, there was no statistically significant difference between the radiographic success of MTA and FC.
Conclusion: White MTA showed a higher clinical and radiographic success rate when compared to FC as a pulpotomy agent in vital primary molars, and it has a potential to become a replacement for FC in primary molars.

Keywords: Formocresol, mineral trioxide aggregate, primary molars, pulpotomy


How to cite this article:
Olatosi O O, Sote E O, Orenuga O O. Effect of mineral trioxide aggregate and formocresol pulpotomy on vital primary teeth: A clinical and radiographic study. Niger J Clin Pract 2015;18:292-6

How to cite this URL:
Olatosi O O, Sote E O, Orenuga O O. Effect of mineral trioxide aggregate and formocresol pulpotomy on vital primary teeth: A clinical and radiographic study. Niger J Clin Pract [serial online] 2015 [cited 2019 Nov 15];18:292-6. Available from: http://www.njcponline.com/text.asp?2015/18/2/292/151071


   Introduction Top


Dental caries and traumatic injuries can cause the dental pulp to undergo irreversible damage which can lead to premature loss of a tooth. One of the major goals in pediatric dentistry is to maintain the primary dentition in an intact state until the permanent successors erupt. [1],[2]

Pulpotomy is a common treatment done to retain cariously involved primary molars that would otherwise be extracted. In pulpotomy, the coronal part of the pulp is removed, and the remaining pulp tissue is covered with a medicament to maintain the vitality of the radicular pulp. [3] The pulpotomy procedure is based on the rationale that the radicular pulp tissue is healthy or is capable of healing after surgical amputation of the affected or infected coronal pulp. [4]

Although many materials such as FC, ferric sulfate, glutaraldehyde, calcium hydroxide, zinc oxide eugenol (ZOE) have been used over the years for pulpotomy procedure, the ideal material should meet certain requirements such as being bactericidal, harmless to the pulpal tissue and surrounding structures, promoting healing of the radicular pulp, not interfering with normal physiological root resorption, and preserving the radicular pulp without any clinical or radiographic symptoms. [1] Formocresol (FC) has been a popular pulp medicament most frequently used in clinical practice for pulpotomy in primary teeth. Doubts have arisen as to the safety of FC in spite of its efficacy. [5],[6],[7],[8] These findings have led to the search for a suitable alternative to replace FC. This replacement medicament must be equally effective but without the undesirable side-effects of FC.

Mineral trioxide aggregate (MTA) was developed by Torabinejad at Loma Linda University in 1995. Mineral trioxide aggregate was introduced as a potential alternative dressing material for pulpotomy of primary molars with features of stimulating cytokine release from bone cells, inducing hard tissue formation, a dentinogenic effect on the pulp, antimicrobial properties, and ability to maintain pulp integrity after pulp capping and pulpotomy without cytotoxic effect. [9]

The aim of this study, therefore, was to evaluate and compare the clinical and radiographic response of FC and MTA as pulpotomy materials on primary molars.


   Materials and Methods Top


Study design and sampling

The study was carried out on primary molars of children aged 4-7 years selected from patients referred to the Pediatric Dentistry unit of the Department of Child Dental Health Lagos University Teaching Hospital with good general health and no history of systemic illness or hospitalization.

The study was carried out on 50 primary molars requiring pulpotomy treatment. Each tooth was randomly assigned to one of the two treatment groups, the FC and MTA group.

The teeth requiring pulpotomy were selected according to the following clinical criteria:

  • Deep carious lesion that exposes vital pulp but symptomless
  • Absence of clinical symptoms or evidence of the pulp degeneration, such as swelling (pulpal origin), sinus tract, tenderness to percussion, pathologic tooth mobility, excessive bleeding from amputated radicular pulp stumps
  • Possibility of proper restoration with Stainless steel crown
  • No radiographic evidence of the pulp degeneration such as, internal and external resorption, inter-radicular, and/or periapical bone destruction or pulp stones.
Ethics

An approval for the research was obtained from the Health Research and Ethics Committee of the Lagos University Teaching Hospital. Informed consent was obtained from parents/guardians. Participation in the study was voluntary.

Pulpotomy procedure

All pulpotomy procedures were performed by a pediatric dentist. Local anesthesia was administered, and the teeth were isolated with a rubber dam. After removal of the carious lesion, the pulp chamber was accessed with bur N°330, high-speed handpiece and water spray. Following removal of the coronal pulp with a slow round bur, hemostasis was obtained with moistened cotton pellets. The medicaments used were FC (Cresol Formalina GHIMAS S.P.A Italy) and MTA (White Proroot MTA, DENTSPLY Tulsa Dental, USA). In the FC group, cotton pellet dipped in 1:5 diluted FC (Buckley's solution) of which excess was squeezed out and placed on the pulp stump for 5 min.

In the MTA group, white MTA was prepared according to the manufacturer's instruction by mixing MTA powder with distilled water at a 3:1 powder/water ratio and placed over the pulp stump. In both groups, ZOE cement was placed to fill the pulp chamber. The teeth were restored with stainless steel crown (SSC 3M ESPE) at the same visit. Clinical and radiographic evaluations of each tooth were performed at 1, 3, 6, 9, and 12 months after the pulpotomy.

At each follow-up appointment, the pulpotomy was considered clinically successful if the treated primary molars showed the following: No symptoms of pain, no tenderness to percussion, no swelling or sinus tract, no pathologic tooth mobility. The pulpotomy was considered radiographically successful at follow-up appointment if the radiograph showed the following: A normal periodontal ligament space, no furcation or periapical radioluscency, no active/progressing internal root resorption, no pathologic external root resorption. Deviation from any one of these clinical and/or radiographic signs was regarded as a failure of treatment.

At each follow-up visit, clinical assessment of the treated teeth which were only identified by code was carried out by two experienced dentists who were blinded to the treatment groups. Series of preoperative and postoperative radiographs for each treated tooth were independently evaluated by the dentists and were both calibrated by evaluating a series of randomly selected pre- and post-operative radiographs from ten children. The intra and inter-examiner reproducibility were calculated by Cohen's unweighted Kappa statistic and were 0.94 and 0.97, respectively.

Statistical analysis

Statistical analysis was carried out using Epi info 7 Centers for Disease Control and Prevention (CDC) Antlanta, Geogia (USA). Description of the patients and teeth included in this study was tabulated. In addition, clinical and radiographic evaluation results were tabulated according to the recall. The differences between the two during the observation period were statistically analyzed using the t-test, Fisher exact test and Chi-square. The level of significance was set at P < 0.05.


   Results Top


A total of 50 vital primary molars in 37 child patients was used in the study, with an equal number of teeth in both the FC and MTA groups. The age range for the patients was 4-7 years in both groups. A total number of four teeth in two patients was lost to follow-up at the end of the 12 months evaluation period [Table 1].
Table 1: Number of pulpotomized teeth and follow - up times according to agents


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Clinical and radiographic success rates of MTA and FC were 100% at 1, 3, and 6 months with no signs of failure.

The number of teeth evaluated at 9 months follow-up was 49. All the 25 teeth in the MTA group were available for follow-up and did not show any sign/symptom of failure. Of the 24 teeth available in the FC group, 1 (4.2%) tooth had failed due to pathological tooth mobility. The remaining 23 (95.8%) treated teeth showed no signs/symptoms of pain, tenderness to percussion, swelling and/or sinus tract and pathologic tooth mobility. Clinical success rate at 9 months was 100% and 95.8% for MTA and FC, respectively, with no statistically significant difference between the two agents (P = 0.49) [[Table 2]a].
Table: 2

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The radiographic success rate at 9 months for the FC group was 83.3% and 100% for MTA. This difference was statistically significant (P = 0.05) [[Table 2]b]. Of the 24 teeth available in the FC group, 20 (83.3%) teeth showed no signs of failure, while 4 (16.8%) showed signs of radiographic failure. Findings in the teeth with radiographic failure in the FC group were 1 (4.2%) internal root resorption, 1 (4.2%) external root resorption, 1 (4.2%) furcation radiolucency and 1 (4.2%) periodontal ligament space widening. The tooth with periodontal ligament space widening also showed signs of furcation radiolucency.

At 12 months follow-up period, 46 teeth were available for evaluation of which 25 were for the MTA group and 21 for the FC group. In the FC group 17 (81%) teeth had no signs and symptoms. A total of four had failed, three due to buccal sinus and one due to pathological tooth mobility. There was no sign of clinical failure in the MTA group. The clinical success rate at 12 months was 100% and 81% for MTA and FC, respectively. This finding was statistically significant (P = 0.04) [[Table 3]a].
Table: 3

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The radiographic evaluation at 12 months in the MTA group 24 (96%) teeth showed no signs of failure, but failure was observed in 1 (4%) tooth with furcation involvement. In the FC group 17 (81%) teeth showed no signs of radiographic failure. A total of 4 (19%) teeth showed signs of radiographic failure. Of these four teeth, 2 (9.5%) had furcation involvement, 1 (4.8%) had external root resorption, and 1 (4.8%) had external root resorption and internal root resorption [Figure 1] in the same tooth. The radiographic success rates for MTA and FC were 96% and 81%, respectively. There was no statistically significant difference (P = 0.20) between the two agents [[Table 3]b].
Figure 1 : (a). preoperative radiograph of FC treated tooth (b) 6 months follow-up (no signs of failure) (c) 9 months follow-up (d) At 12 months follow-up (internal root resorption)

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At the end of the 12 months follow-up, the clinical success rates for FC and MTA were 81% and 100% respectively. There was a statistically significant difference (P = 0.04) between the clinical success rates of FC and MTA. While the radiographic success rates for FC and MTA were 81% and 96%, respectively, there was no statistically significant difference between the radiographic success of MTA and FC [[Table 4]].
Table 4: Clinical and radiographic assessment of MTA and FC treated primary molars at different evaluation times


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   Discussion Top


The present study was conducted to evaluate the clinical and radiographic success rates of pulpotomies performed using MTA a relatively new agent in endodontics, which has been demonstrated to be biocompatible, nontoxic, promote regeneration of pulp tissues and which fulfills the purpose of pulpotomy treatment; to maintain the treated teeth in a functional state. [10],[11],[12]

Formocresol was used as a control group since it is still the gold standard in primary teeth pulp therapy, in spite of its reported toxic, mutagenic, and carcinogenic properties. [5],[6],[7],[8] It is possible that FC would be abandoned if an effective nontoxic alternative is identified.

The ages of the children ranged between 4 and 7 years due to lack of cooperation that may be seen in children <4 years and the possibility of physiologic root resorption (>3/4 of root) in children above the age of 7 years. [2],[13]

The success of any clinical procedure is usually determined during the follow-up visits. Availability of patients at scheduled follow-up appointments is, therefore, an important factor which must be considered in determining the efficacy of the treatment. [11]

In this study, 37 children had pulpotomy done on 50 primary molars. A total of 46 teeth in 35 children was available for evaluation after 12 months. This high number of patients available for follow-up was probably due to a good rapport with the children, their parents and repeated reminders. The two children lost to follow-up were as a result of relocation to another city and loss of interest.

Our findings showed a higher success rate in teeth treated with MTA (100% clinical, 96% radiographic) than those with FC (81% clinical, 81% radiographic) throughout the follow-up period both clinically and radiographically. There was a statistically significant difference between the clinical success rates of MTA and FC (P = 0.04) at the end of the 12 months follow-up period. These findings are similar to those reported by other authors. [11],[14],[15] The lower success rate for FC compared with MTA observed in this study is consistent with previous studies that showed decreased success rate of FC with time. [16],[17],[18]

The common radiographic failure in the present study in the FC group were internal/external root resorptions, inter radicular/periapical radiolucency, while the clinical failures in the FC group, where the presence of sinus and pathologic tooth mobility. The probable reason for this may be due to the fixative effect of FC and its ability to vaporize and escape through the apical foramen. In the MTA group, there was no clinical failure, the radiographic finding of inter radicular radiolucency seen in one tooth may be attributed to misdiagnosis of inflammation in the radicular pulp prior to treatment.

Internal root resorption was observed in this study in one tooth (4.2%) in the FC group both at 9 months and 12 months follow-up evaluation and none in the MTA group. This agrees with the findings by Eidelman et al. who reported one case (7%) of internal root resorption in the FC group and none in the MTA group. [15] Holan et al. also found 4 teeth (13.8%) in the FC group with internal root resorption which was associated with periapical and/or inter-radicular radiolucent defects and external root resorption. [11] There are reports that internal resorption is associated with eugenol. [19] When used together with zinc oxide (as a sub-base following pulpotomy), eugenol comes into direct contact with vital tissue following hydrolysis of zinc eugenol to yield the free eugenol. This causes a moderate to severe inflammatory response, resulting in chronic inflammation and necrosis. It has been reported that internal resorption is more likely the result of undiagnosed chronic inflammation existing in the radicular pulp prior to pulpotomy than the result of exposure of the radicular pulp to eugenol. [11] Smith et al. also claimed that the fixed layer caused by FC on the pulp serves as a barrier to eugenol. [19]

The internal root resorption seen in this study is not likely to come from eugenol as ZOE paste was placed as a base on FC fixed tissue which serves as a barrier to eugenol, rather the internal root resorption may be from the reversible fixative effect of FC and its irritative pH.

In the present study, there was a statistically significant difference in the success rate using clinical criteria, with MTA showing better outcomes at the end of the 12 months evaluation period when compared with FC.

The greater success rate of MTA can be attributed to its biocompatibility and sealing ability when compared to FC.

Despite the promising findings regarding the use of MTA in this study, it is important to note that the follow-up period was short. There is, therefore, a need to conduct further studies with a larger sample size and longer follow-up period. It is also important to study and determine the possible effects of MTA on succedaneous permanent teeth. Further, histological studies are also needed to determine the pulpal response to this material. [20] However, the results here indicate that MTA has a promising potential to be a replacement for FC after pulpotomy in primary molars.


   Conclusion Top


Mineral trioxide aggregate showed clinical and radiographic success as a dressing material following pulpotomy procedure in primary teeth, and it has a promising potential to become a replacement for FC in primary molars. Though FC has been a controversial medicament for decades, it is still being used for pulpotomy procedure. However, MTA may be a potential replacement for FC. One of the limitations of MTA is its high cost and technique sensitivity.

 
   References Top

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Eidelman E, Holan G, Fuks AB. Mineral trioxide aggregate vs. formocresol in pulpotomized primary molars: A preliminary report. Pediatr Dent 2001;23:15-8.  Back to cited text no. 15
    
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    Figures

  [Figure 1]
 
 
    Tables

  [Table 1], [Table 2], [Table 3], [Table 4]


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