|Year : 2018 | Volume
| Issue : 6 | Page : 703-710
Is Low-level laser therapy and gaseous ozone application effective on osseointegration of immediately loaded implants?
IR Karaca1, G Ergun2, DN Ozturk1
1 Department of Oral and Maxillofacial Surgery, Gazi University, Faculty of Dentistry, Ankara, Turkey
2 Department of Prosthodontics, Gazi University, Faculty of Dentistry, Mersin, Turkey
|Date of Acceptance||01-Nov-2017|
|Date of Web Publication||11-Jun-2018|
Prof. I R Karaca
Department of Oral and Maxillofacial Surgery, Gazi University, Faculty of Dentistry, Biskek Cd (8.cd) 82. Sok. No: 4 06510 Emek-Ankara
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Purpose: The purpose of this study was to investigate the effects of biostimulation lasers and ozone therapy on osseointegration of immediately loaded implants. Materials and Methods: A total number of 100 implants (DTI Implant Systems) were applied to 25 patients evenly. Temporary crowns were applied to each patient on the same session as the surgery. Implants were divided into four treatment groups (Group 1: low-level laser therapy (LLLT) group, Group 2: ozone therapy group, Group 3: different protocol of ozone therapy group, and Group 4: control group) each with 25 implants. The irradiations were performed with a gallium-aluminum-arsenide diode low-level laser (Laser BTL-4000) to Group 1. Ozone therapy was performed using an ozone generator (OzoneDTA) with an intraoral probe to Group 2 and Group 3. Results: In this study, the overall implant survival rate was 92% after a 6-month observation period. The implant stability quotient values were found significantly higher in Group 1 (LLLT group) and Group 3 (different protocol of ozone therapy group) than the other groups (P < 0.05). There was no significant difference in Group 2 (ozone therapy group) and the control group (P > 0.05). Conclusions: Our results suggest that both LLLT and ozone therapy with prolonged application time are promising methods to enhance bone healing around immediately loaded implants and increase implant stability; however, there is a need for more studies on this subject for these methods to become routine applications.
Keywords: Immediate loading, implant stability, laser therapy, ozone therapy, resonance frequency analysis
|How to cite this article:|
Karaca I R, Ergun G, Ozturk D N. Is Low-level laser therapy and gaseous ozone application effective on osseointegration of immediately loaded implants?. Niger J Clin Pract 2018;21:703-10
|How to cite this URL:|
Karaca I R, Ergun G, Ozturk D N. Is Low-level laser therapy and gaseous ozone application effective on osseointegration of immediately loaded implants?. Niger J Clin Pract [serial online] 2018 [cited 2018 Oct 22];21:703-10. Available from: http://www.njcponline.com/text.asp?2018/21/6/703/234051
| Introduction|| |
The immediate loading (IL) technique has been widely used in oral implant rehabilitations to reduce treatment time. This technique eliminates the necessity to wait during the healing time, permits the use of provisional prostheses after implant insertion, and keeps the implants in function during the healing process. However, some trends suggest that immediately loaded implants have lower survival rates than conventionally loaded implants., Success of IL depends on various factors such as primary stability, surgical technique, bone quality, systemic diseases, number of implants, and occlusal forces. Primary stability is one of the most important variables in IL. Resonance frequency analysis (RFA) is the most common method for the evaluation of primary stability. as the implant stability quotient (ISQ) that can range from 0 to 100. Higher ISQ values indicate the stability of an implant to be better. It has also been suggested that ISQ values can be used to evaluate the stiffness of the implant-to-bone interface for IL. Suggested criteria for IL include an ISQ of 50–65, as determined by RFA. Recent studies have been focusing on bone healing. According to these studies, bone healing promotion has been achieved with physical stimuli, such as ultrasound, low-level laser therapy (LLLT), and ozone therapy.,
LLLT is a noninvasive treatment modality that uses low-level (low-power) lasers or light emitting diodes.,, LLLT is known to improve bone healing. In medicine, LLLT has been used for various effects such as biostimulation of wounds, collagen synthesis, and fibroblast proliferation.,, Most studies focused on this subject to evaluate the biostimulation effect of lasers on osteoblast proliferation, collagen deposition, and bone formation compared to bone tissues that have not been exposed to laser light.,, In the literature, there is insufficient data considering these effects of lasers on osseointegration of dental implants.
Ozone therapy is another noninvasive treatment modality used for bone healing. Ozone is naturally present in gaseous form that consists of three atoms of oxygen, but it may also be in aqueous form., Ozone therapy may lead to several effects on the human body. It can enhance blood circulation, and thus oxygen delivery to ischemic tissues. Ozone therapy also plays a role in increasing of cellular antioxidant enzyme and growth factor levels or activation of the immune system.,
Most studies addressing ozone therapy in dentistry has evaluated antimicrobial effects of ozone.,,, However, we have discovered through a broad search of the literature that there are no studies considering the use of ozone therapy in dental implants. Since ozone is known to have therapeutic effects on wound healing by improving blood supply, upregulation of antioxidant enzymes and growth factors and activation of the immune system; we hypothesized that ozone therapy may improve the stability and predictability of implants by promoting bone healing.
Our hypothesis was that laser and ozone therapies will enhance bone healing around immediately loaded implants. The aim of the present study is to test this hypothesis on enhancing the osseointegration of immediately loaded dental implants using biostimulation lasers and ozone therapy.
| Materials and Methods|| |
Patient selection and study design
In this study, data were collected in Gazi University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery and Department of Prosthodontics between January 2016 and December 2016. The protocol of this study was approved by the Ethical Committee of Gazi University. The patients were informed in detail about the surgical procedure and were instructed about postoperative care, follow-up examinations, and alternative treatment options available to them. Informed written signed consent was obtained from all participants according to the Declaration of Helsinki.
Inclusion criteria were as follows – patients with a noncontributory medical history, patients older than 18 years of age, adequate amount of bone height (minimum 12 mm above the mandibular canal), and width (minimum 5.0 mm) for placement of implants, implant site with no signs of acute infection.
The following exclusion criteria were applied – patients under 18 years of age, patients who were nursing or pregnant, if the treatment could somehow affect the patient's health condition (diabetic patients and smokers were excluded), patients whose cooperation appeared questionable, patients whose systemic condition was not suitable for ozone and LLLT or patients who did not give his/her informed consent to participate.
A total number of 100 implants (DTI Implant Systems, Istanbul, Turkey) were applied in 25 patients who referred to our department with proper indications. Four dental implants (with a diameter of 4 or 4.5 mm and a length of 10 mm) were placed in the posterior mandible of each patient. Dental implants placed in proper locations and angulations in the posterior mandible.
Implants were divided into four treatment groups, each with 25 implants (three study groups [Group 1: LLLT, Group 2: ozone therapy, and Group 3: different protocol of ozone therapy) and a control group (no LLLT or ozone therapy]).
All patients were subjected to a standardized surgical protocol, ozone and laser therapy by a surgeon with 25 years of experience. Another surgeon with 4 years of experience carried out the measurements and was blind to the groups of patients included. Pain levels of the patients were determined by visual analog scale (VAS) postoperatively and at 1, 3, 5, and 7 days after surgery. Plaque index and bleeding on probing scores were also evaluated on the 3rd and 6th months postoperatively.
After administering local anesthesia, the initial surgical incision was made, and a mucoperiosteal flap was elevated. By means of a surgical guide, appropriate implant positions were marked with a round bur. Drills of increasing diameters were used in preparing the implant site. Proper-sized implants were placed to the correct depth with the final placement torque values of 35–50 N cm. All implants were placed using the nonsubmerged technique. The flaps were sutured with 3-0 silk sutures (Dogsan 3.0, 16 mm, 3/8, cutting suture, Trabzon, Turkey). Patients were informed with postsurgical instructions. Postoperatively, the patients were prescribed 1000 mg amoxicillin and 25 mg dexketoprofen orally and 0.2% chlorhexidine mouth rinse (1 min, three times daily) every day for 1 week. After implant placement, immediate prosthetic loading was performed.
Immediately after implant placement, provisional fixtures for the four treatment groups were delivered as single-unit crowns that were slightly out of occlusion.
After implant placement, single screw-retained provisional acrylic (Telio CS, C and B [A2] Ivoclar, Vivadent Amherst, NY, USA) crowns were fixed over the four treatment group implants 2 h after surgery. The titanium abutments and provisional crowns were screwed as one unit and tightened to 10 N cm.
Provisional crowns were adjusted to any vertical load or proximal contact for slight occlusion or articulation on the implants.
Six months following implant placement, the final impressions were taken (Impregum 3M ESPE, St. Paul, MN, USA), within 2 weeks after impression taking, permanent screw-retained implant-supported metal-ceramic crowns were placed on the same titanium abutments. The titanium abutments' screws were torqued in place to 30 N cm so that cement failure was avoided.
Resonance frequency analysis
A wireless magnetic-based Osstell Mentor RF Analyzer (Osstell USA, Linthicum, MD, USA) was used to evaluate primary implant stability. The designated transducer (SmartPeg) was tightened to the fixture. Three measurements were done for both buccal and lingual sites. From these three measurements, the mean value was obtained for one site (buccal or lingual), and as a result, two ISQ values were recorded to assess the stability of each implant. RFA measurements were done immediately after the surgery and 6 months postoperatively. The screw-retained provisional acrylic crowns were removed for the 6th-month measurements and replaced with the final prostheses after. Prognoses of dental implants in the study and control groups were investigated by these measurements.
Low-level laser therapy
The irradiations were performed with a gallium-aluminum-arsenide diode low-level laser with continuous emission of 830-nm wavelength (Laser BTL- 4000, Brno, Czech Republic). The laser power was of 86 ± 2 mW and the laser spot size was 0.0028 cm2, resulting in a calculated energy density of 92.1 J/cm2, and an energy of 0.25 J per point. The irradiation time was three seconds per point, in contact. The total delivered energy was 5 J, equally divided by 20 irradiation points. The first irradiation was performed in the immediate postoperative period at 20 points: nine points at the vestibular region, nine at the lingual, one at the distal, and one at the mesial region of the implant. The LLLT was repeated every two days for 2 weeks [Figure 1].
Ozone therapy was performed using an ozone generator (OzoneDTA; Apoza, Taiwan, ROC) with an intraoral probe according to information given by the manufacturer. The ozone generator was applied intraorally with an intensity of 80% for 3 min in Group 2 and 6 min in Group 3 patients, three times a week for 2 weeks [Figure 2].
The data obtained in this study were analyzed by SPSS (IBM Corp. Released 2011. IBM SPSS Statistics for Windows, Version 20.0. Armonk, NY: IBM Corp). After the data were subjected to normality tests, Wilcoxon-signed rank test was used to assess statistically significant differences among the parameters within the groups, and Kruskal–Wallis H test was used for group comparisons. The level of significance was set at 0.05. There was no significant difference when P > 0.05 and there was a significant difference when P < 0.05.
| Results|| |
This study involved 25 patients (11 males and 14 females) with bilaterally edentulous posterior mandible, and their mean ± standard deviation age was 51.2 ± 2.3 years (range 36–64 years). A total of 100 dental implants were inserted without any complication. In this study, the overall implant survival rate was 92% after a 6-month observation period. Eight implants failed for unknown reasons, and as a consequence of these failures, new implants were placed to the same regions after adequate bone healing occurred. All the other implants and related prostheses were stable, and no complication was observed during the follow-up period.
Patients were recalled postoperatively on days 1, 3, 5, and 7 and evaluated for pain. There was no significant difference between study groups and the control group on VAS scale [Table 1]. Plaque index and bleeding on probing scores at the 3rd and 6th months showed no significant difference between study groups and the control group [Table 2] and [Table 3].
|Table 1: Comparison of postoperative visual analog scale scores between groups|
Click here to view
|Table 2: Comparison of plaque scores between groups at different observation times|
Click here to view
|Table 3: Comparison of bleeding on probing scores between groups at different observation times|
Click here to view
In the present study, the level of primary implant stability exceeded 65 ISQ in all study and control groups. The lowest measured value of primary implant stability was 68 ISQ in 100 implants. Over the observation time, the implant stability increased in all groups. The ISQ values were found significantly higher in the Group 1 and Group 3 than the other groups after 6-months (P< 0.05). There was no significant difference between Group 2 and the control group at the 6th-month follow-up [P > 0.05] [Table 4].
|Table 4: Comparison of implant stability quotient values between groups at different observation times|
Click here to view
| Discussion|| |
Many studies have reported promising outcomes of LLLT and ozone therapy regarding the bone-healing process; still, little data exist relating to these effects on implant osseointegration.,,,,, The aim of this study was to evaluate the effect of LLLT and ozone therapy on immediately loaded implant osseointegration.
Implant stability has been assessed by RFA in many studies.,,, Although, ISQ values are not a perfectly reliable source of information for implant success; it is widely used in the literature.,, Therefore, RFA was used to measure implant stability in this study. Recent data indicate that an ISQ value of primary implant stability between 60 and 65 is sufficient for IL.,, In the present study, primary implant stability was sufficient in all study groups. The lowest measured value of primary implant stability was 68 ISQ in 100 implants.
In this study, the overall implant survival rate was 92% after a 6-month observation period. Eight implants failed in this period for unknown reasons, and as a consequence of this failure, new implants were placed to the same regions after adequate bone healing was obtained. All the other implants and related prostheses were stable, and no complication was observed during the follow-up period. In the literature, immediately loaded implants showed a high survival rate (96.8%) in the posterior part of the mandible as in this study (92%).,, During the observation time, the implant stability increased in all survived implants. The ISQ values were found significantly higher in the LLLT group than the other groups after 6 months. This increase in osseointegration could be referred to an improvement in the bone-healing phase around the implants. There are several studies that focus on IL in posterior maxilla which report lower survival rates when compared to conventionally loaded implants.,, However, the standardized protocol of this study only included patients with bilaterally edentulous mandibles; a different study may be planned to observe the effects of LLLT and ozone therapy on immediately loaded implants in the maxilla.
The use of lasers to achieve biostimulation for bone regeneration has become popular in medicine, and many researchers have reported that LLLT has positive effects on bone healing.,,,, LLLT has an anti-inflammatory effect through several mechanisms such as induction of ascorbic acid uptake by cells, enhancement of mitochondrial adenosine triphosphate production, stabilization of the cell membrane, lymphocyte stimulation, mast cell activation, and proliferation of various cell types. It also promotes local cell circulation, cell proliferation, and collagen synthesis. LLLT is known to demonstrate biomodulary effect of the laser light by regulating cell physiology or stimulating the proliferation and differentiation of undifferentiated cells. This information leads us to think that it may also enhance the bone healing process.
There are several variations in the literature regarding the choice of the power, density, and wavelength when using low energy level laser therapy in bone healing.,,,,,,,, Khadra et al. reported that a standard protocol for laser irradiation in dental implant procedures has not yet been identified. It has been suggested that the 830 nm wavelength laser has a positive effect on the recovery of both fibroblasts and osteoblasts by different authors.,,,,, García-Morales et al. used 830 nm wavelength, at 86 mW power and 92.1 J/cm2 density. The laser settings chosen in this study were based on clinical trials, in which positive effects of LLLT on wound healing after wisdom tooth extraction and bone healing after dental implant application were shown.,
In the many studies that determine adjunctive therapies to the bone healing mechanisms, LLLT promotes the healing process in bone defects.,,,,, Garcia et al. have reported that the diode laser is effective in inducing bone formation in the calvarium defects of osteoporosis-induced rats. Likewise, Pretel et al. and Fávaro-Pípi et al. reported that LLLT administration had positive effects on bone healing. In another study, laser application is found to accelerate fracture repair process by providing increased callus volume and bone mineral density. Renno et al. reported that laser application at a wavelength of 830 nm provided a significant increase in the proliferation of osteoblasts. Sella et al. found that low-level laser application to fractured rat femurs increased the amount of bone tissue in the fractured area. In the present study, ISQ values revealed a significant increase in low-level laser therapy group at 6 months. This result was consistent with the results of those studies mentioned above,,,,,, and low-level laser therapy was found to have a positive effect on the stability of dental implants.
Clinical evidence for ozone application in dentistry is not adequate. Ozone therapy has positive effects on oxygen metabolism, cell energy, immune system, antioxidant defense system, and microcirculation. In this study, ozone therapy was applied to two different groups for different durations. In Group 2, a protocol used in a previous study  that indicate a positive effect of ozone therapy on wound healing after tooth extraction was utilized. Another protocol of ozone therapy with a longer application time was described in the user guide of the ozone device as applicable to oral mucosa, and this protocol was used in Group 3. A statistically significant increase in ISQ values was observed in Group 3 at the end of 6 months compared to Group 2 and the control group. No statistically significant difference was found between Group 2 and the control group. According to this result, it is observed that maximum dose of ozone application had a positive effect on implant stability, but no comparison was made in the literature since there is no study evaluating the effect of ozone application on osseointegration.
It is important to point out that this study is limited to one anatomic site, a single type of implant, a particular type of prosthetic appliance (single crowns) and a specific methodology, and results may differ in different anatomic sites (maxilla), prosthetic appliances (dentures or bridges) and implants when using different LLLT, and ozone therapy protocols with other methodologies and different lengths of follow-up. Thus, more studies similar to this one should be planned and conducted with different methodology to contribute to the literature.
Within the limitations of this study, it is concluded that both laser therapy and ozone therapy with prolonged application time increase implant stability in the immediate slightly functional loading of single implants in the posterior mandible. However, the standard application of ozone did not seem to have any effect on implant stability. Our data suggest that both ozone and laser therapies provide additional new insights into therapeutic strategies in improving implant stability in dentistry, but further experimental and clinical evaluations are needed.
We would like to thank Dr. Elif Peker and Dr. Faruk Ogutlu for their assistance in the surgical procedures.
Financial support and sponsorship
This study was supported by a Grant from Gazi University Scientific Research Foundation (03/2016.11).
Conflicts of interest
There are no conflicts of interest.
| References|| |
Gao J, Matsushita Y, Esaki D, Matsuzaki T, Koyano K. Comparative stress analysis of delayed and immediate loading of a single implant in an edentulous maxilla model. J Dent Biomech 2014;5:1-7.
Al-Sawai AA, Labib H. Success of immediate loading implants compared to conventionally-loaded implants: A literature review. J Investig Clin Dent 2016;7:217-24.
Kim SJ, Ribeiro AL, Atlas AM, Saleh N, Royal J, Radvar M, et al.
Resonance frequency analysis as a predictor of early implant failure in the partially edentulous posterior maxilla following immediate nonfunctional loading or delayed loading with single unit restorations. Clin Oral Implants Res 2015;26:183-90.
Rismanchian M, Attar BM, Razavi SM, Shamsabad AN, Rezaei M. Dental implants immediate loading versus the standard 2-staged protocol: An experimental study in dogs. J Oral Implantol 2012;38:3-10.
Wentaschek S, Scheller H, Schmidtmann I, Hartmann S, Weyhrauch M, Weibrich G, et al.
Sensitivity and specificity of stability criteria for immediately loaded splinted maxillary implants. Clin Implant Dent Relat Res 2015;17 Suppl 2:e542-9.
Kazancioglu HO, Ezirganli S, Aydin MS. Effects of laser and ozone therapies on bone healing in the calvarial defects. J Craniofac Surg 2013;24:2141-6.
Nagata MJ, Santinoni CS, Pola NM, de Campos N, Messora MR, Bomfim SR, et al.
Bone marrow aspirate combined with low-level laser therapy: A new therapeutic approach to enhance bone healing. J Photochem Photobiol B 2013;121:6-14.
Avci P, Gupta A, Sadasivam M, Vecchio D, Pam Z, Pam N, et al.
Low-level laser (light) therapy (LLLT) in skin: Stimulating, healing, restoring. Semin Cutan Med Surg 2013;32:41-52.
Chung H, Dai T, Sharma SK, Huang YY, Carroll JD, Hamblin MR, et al.
The nuts and bolts of low-level laser (light) therapy. Ann Biomed Eng 2012;40:516-33.
García-Morales JM, Tortamano-Neto P, Todescan FF, de Andrade JC Jr., Marotti J, Zezell DM, et al.
Stability of dental implants after irradiation with an 830-nm low-level laser: A double-blind randomized clinical study. Lasers Med Sci 2012;27:703-11.
Pourzarandian A, Watanabe H, Ruwanpura SM, Aoki A, Ishikawa I. Effect of low-level er:YAG laser irradiation on cultured human gingival fibroblasts. J Periodontol 2005;76:187-93.
Eduardo FP, Mehnert DU, Monezi TA, Zezell DM, Schubert MM, Eduardo CP, et al.
Cultured epithelial cells response to phototherapy with low intensity laser. Lasers Surg Med 2007;39:365-72.
Fujihara NA, Hiraki KR, Marques MM. Irradiation at 780 nm increases proliferation rate of osteoblasts independently of dexamethasone presence. Lasers Surg Med 2006;38:332-6.
da Silva RV, Camilli JA. Repair of bone defects treated with autogenous bone graft and low-power laser. J Craniofac Surg 2006;17:297-301.
Bocci VA. Scientific and medical aspects of ozone therapy. State of the art. Arch Med Res 2006;37:425-35.
Ozdemir H, Toker H, Balcı H, Ozer H. Effect of ozone therapy on autogenous bone graft healing in calvarial defects: A histologic and histometric study in rats. J Periodontal Res 2013;48:722-6.
Sagai M, Bocci V. Mechanisms of action involved in ozone therapy: Is healing induced via a mild oxidative stress? Med Gas Res 2011;1:29.
Polydorou O, Halili A, Wittmer A, Pelz K, Hahn P. The antibacterial effect of gas ozone after 2 months ofin vitro
evaluation. Clin Oral Investig 2012;16:545-50.
Huth KC, Jakob FM, Saugel B, Cappello C, Paschos E, Hollweck R, et al.
Effect of ozone on oral cells compared with established antimicrobials. Eur J Oral Sci 2006;114:435-40.
Polydorou O, Pelz K, Hahn P. Antibacterial effect of an ozone device and its comparison with two dentin-bonding systems. Eur J Oral Sci 2006;114:349-53.
Kazancioglu HO, Ezirganli S, Demirtas N. Comparison of the influence of ozone and laser therapies on pain, swelling, and trismus following impacted third-molar surgery. Lasers Med Sci 2014;29:1313-9.
Agrillo A, Ungari C, Filiaci F, Priore P, Iannetti G. Ozone therapy in the treatment of avascular bisphosphonate-related jaw osteonecrosis. J Craniofac Surg 2007;18:1071-5.
Petrucci MT, Gallucci C, Agrillo A, Mustazza MC, Foà R. Role of ozone therapy in the treatment of osteonecrosis of the jaws in multiple myeloma patients. Haematologica 2007;92:1289-90.
Bischof M, Nedir R, Szmukler-Moncler S, Bernard JP, Samson J. Implant stability measurement of delayed and immediately loaded implants during healing. Clin Oral Implants Res 2004;15:529-39.
Nedir R, Bischof M, Szmukler-Moncler S, Bernard JP, Samson J. Predicting osseointegration by means of implant primary stability. Clin Oral Implants Res 2004;15:520-8.
Glauser R, Sennerby L, Meredith N, Rée A, Lundgren A, Gottlow J, et al.
Resonance frequency analysis of implants subjected to immediate or early functional occlusal loading. Successful vs. Failing implants. Clin Oral Implants Res 2004;15:428-34.
Sim CP, Lang NP. Factors influencing resonance frequency analysis assessed by osstell mentor during implant tissue integration: I. Instrument positioning, bone structure, implant length. Clin Oral Implants Res 2010;21:598-604.
Monje A, Ortega-Oller I, Galindo-Moreno P, Catena A, Monje F, O'Valle F, et al.
Sensitivity of resonance frequency analysis for detecting early implant failure: A case-control study. Int J Oral Maxillofac Implants 2014;29:456-61.
Krafft T, Graef F, Karl M. Osstell resonance frequency measurement values as a prognostic factor in implant dentistry. J Oral Implantol 2015;41:e133-7.
González-García R, Monje F, Moreno-García C. Predictability of the resonance frequency analysis in the survival of dental implants placed in the anterior non-atrophied edentulous mandible. Med Oral Patol Oral Cir Bucal 2011;16:e664-9.
Kokovic V, Jung R, Feloutzis A, Todorovic VS, Jurisic M, Hämmerle CH, et al.
Immediate vs. Early loading of SLA implants in the posterior mandible: 5-year results of randomized controlled clinical trial. Clin Oral Implants Res 2014;25:e114-9.
Vanden Bogaerde L, Pedretti G, Dellacasa P, Mozzati M, Rangert B, Wendelhag I, et al.
Early function of splinted implants in maxillas and posterior mandibles, using branemark system tiunite implants: An 18-month prospective clinical multicenter study. Clin Implant Dent Relat Res 2004;6:121-9.
Vanden Bogaerde L, Pedretti G, Dellacasa P, Mozzati M, Rangert B. Early function of splinted implants in maxillas and posterior mandibles using branemark system machined-surface implants: An 18-month prospective clinical multicenter study. Clin Implant Dent Relat Res 2003;5 Suppl 1:21-8.
Vervaeke S, Collaert B, De Bruyn H. Immediate loading of implants in the maxilla: Survival and bone loss after at least 2 years in function. Int J Oral Maxillofac Implants 2013;28:216-21.
Cricchio G, Imburgia M, Sennerby L, Lundgren S. Immediate loading of implants placed simultaneously with sinus membrane elevation in the posterior atrophic maxilla: A two-year follow-up study on 10 patients. Clin Implant Dent Relat Res 2014;16:609-17.
Pretel H, Lizarelli RF, Ramalho LT. Effect of low-level laser therapy on bone repair: Histological study in rats. Lasers Surg Med 2007;39:788-96.
Khadra M, Rønold HJ, Lyngstadaas SP, Ellingsen JE, Haanaes HR. Low-level laser therapy stimulates bone-implant interaction: An experimental study in rabbits. Clin Oral Implants Res 2004;15:325-32.
Conlan MJ, Rapley JW, Cobb CM. Biostimulation of wound healing by low-energy laser irradiation. A review. J Clin Periodontol 1996;23:492-6.
Pinheiro AL, Gerbi ME. Photoengineering of bone repair processes. Photomed Laser Surg 2006;24:169-78.
Guzzardella GA, Torricelli P, Nicoli-Aldini N, Giardino R. Osseointegration of endosseous ceramic implants after postoperative low-power laser stimulation: Anin vivo
comparative study. Clin Oral Implants Res 2003;14:226-32.
Khadra M, Kasem N, Haanaes HR, Ellingsen JE, Lyngstadaas SP. Enhancement of bone formation in rat calvarial bone defects using low-level laser therapy. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004;97:693-700.
Lopes CB, Pinheiro AL, Sathaiah S, Duarte J, Cristinamartins M. Infrared laser light reduces loading time of dental implants: A Raman spectroscopic study. Photomed Laser Surg 2005;23:27-31.
Lopes CB, Pinheiro AL, Sathaiah S, Da Silva NS, Salgado MA. Infrared laser photobiomodulation (lambda 830 nm) on bone tissue around dental implants: A Raman spectroscopy and scanning electronic microscopy study in rabbits. Photomed Laser Surg 2007;25:96-101.
Sella VR, do Bomfim FR, Machado PC, da Silva Morsoleto MJ, Chohfi M, Plapler H, et al.
Effect of low-level laser therapy on bone repair: A randomized controlled experimental study. Lasers Med Sci 2015;30:1061-8.
Rodrigo SM, Cunha A, Pozza DH, Blaya DS, Moraes JF, Weber JB, et al.
Analysis of the systemic effect of red and infrared laser therapy on wound repair. Photomed Laser Surg 2009;27:929-35.
Gerbi ME, Marques AM, Ramalho LM, Ponzi EA, Carvalho CM, Santos Rde C, et al.
Infrared laser light further improves bone healing when associated with bone morphogenic proteins: Anin vivo
study in a rodent model. Photomed Laser Surg 2008;26:55-60.
Khadra M, Lyngstadaas SP, Haanaes HR, Mustafa K. Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material. Biomaterials 2005;26:3503-9.
Garcia VG, da Conceição JM, Fernandes LA, de Almeida JM, Nagata MJ, Bosco AF, et al.
Effects of LLLT in combination with bisphosphonate on bone healing in critical size defects: A histological and histometric study in rat calvaria. Lasers Med Sci 2013;28:407-14.
Fávaro-Pípi E, Feitosa SM, Ribeiro DA, Bossini P, Oliveira P, Parizotto NA, et al.
Comparative study of the effects of low-intensity pulsed ultrasound and low-level laser therapy on bone defects in tibias of rats. Lasers Med Sci 2010;25:727-32.
Renno AC, McDonnell PA, Parizotto NA, Laakso EL. The effects of laser irradiation on osteoblast and osteosarcoma cell proliferation and differentiation in vitro
. Photomed Laser Surg 2007;25:275-80.
[Figure 1], [Figure 2]
[Table 1], [Table 2], [Table 3], [Table 4]