Medical and Dental Consultantsí Association of Nigeria
Home - About us - Editorial board - Search - Ahead of print - Current issue - Archives - Submit article - Instructions - Subscribe - Advertise - Contacts - Login 
  Users Online: 326   Home Print this page Email this page Small font sizeDefault font sizeIncrease font size
 

  Table of Contents 
ORIGINAL ARTICLE
Year : 2018  |  Volume : 21  |  Issue : 9  |  Page : 1087-1092

Comparison of the mechanical properties of platelet-rich fibrin and ankaferd blood stopper-loaded platelet-rich fibrin


1 Department of Oral Maxillofacial Surgery, Hacettepe University, Faculty of Dentistry, Ankara, Turkey
2 Hacettepe University, Faculty of Chemistry, Polymer Chemistry Division, Ankara, Turkey
3 Department of Bioengineering, Kİrİkkale University, Faculty of Engineering, Kİrİkkale, Turkey
4 Department of Biostatistics, Hacettepe University, Ankara, Turkey

Date of Acceptance15-Mar-2018
Date of Web Publication29-Aug-2018

Correspondence Address:
Dr. A Aktas
Department of Oral Maxillofacial Surgery, Faculty of Dentistry, Hacettepe University, Sihhiye 06100, Ankara
Turkey
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/njcp.njcp_370_17

Rights and Permissions
   Abstract 

Background and Aim: Platelet-rich fibrin (PRF) can be named as a natural fibrin-based biomaterial favorable to increasing vascularization and able to guide epithelial cell migration to its surface. The membrane has a significant positive effect on protecting open wounds and accelerating healing. Similar to PRF Ankaferd Blood Stopper (ABS) also has positive effects on wound healing. The aim of this study was to detect if we can improve known physical properties of PRF combining with ABS. This idea was based on the known mechanism of ABS in forming protein network without damaging any blood cells. Materials and Methods: A total of 25 adult rabbits used for collecting 5–7 ml of blood passively with the help of winged blood collection needle to the test tube. Collected samples were centrifuged at 3000 rpm for 10 min. Two similar samples obtained from each animal and one of the samples was placed in 20% ABS 80% saline solution for 5 min. Mechanical properties of the membrane samples were measured using Universal Testing Machine. Results: There is the statistically significant difference between PRF and ABS added PRF in elongation/mm (dL) and elongation/% at break values. Maximum force (fMax) and modulus values did not show any statistically significant differences. Conclusion: ABS loaded PRF causes better physical properties. This combination seems to exhibit superior performance when used as a membrane barrier solely. Advanced studies can be done on biological properties of ABS loaded PRF, especially on tissue healing.

Keywords: Ankaferd bloodstopper, mechanical properties, platelet-rich fibrin, wound healing


How to cite this article:
Aktas A, Ozer T, Sen M, Turk M, Karabulut E. Comparison of the mechanical properties of platelet-rich fibrin and ankaferd blood stopper-loaded platelet-rich fibrin. Niger J Clin Pract 2018;21:1087-92

How to cite this URL:
Aktas A, Ozer T, Sen M, Turk M, Karabulut E. Comparison of the mechanical properties of platelet-rich fibrin and ankaferd blood stopper-loaded platelet-rich fibrin. Niger J Clin Pract [serial online] 2018 [cited 2018 Nov 15];21:1087-92. Available from: http://www.njcponline.com/text.asp?2018/21/9/1087/240060




   Introduction Top


There are numerous studies about accelerating wound healing. Platelet-rich fibrin (PRF) and platelet rich plasma (PRP) techniques are very popular as a reason of easy accessibility and successful reported studies. PRF technique does not need any gelling agent, which is different from PRP procedure.[1] It is only a centrifuged blood without any addition.[1] Blood sample is taken into a test tube, which does not contain any agent. This sample is centrifuged at 3000 revolutions/min (rpm) for 10 min.[1] Due to the absence of anticoagulant agent in the test tube, platelets come into contact to test tube wall, activating the coagulation cascades. Elapsed time for collecting blood and centrifugation is the main factor for success.[1] During this procedure, PRF polymerizes slowly with the help of thrombin.[1] Fibrin network is formed as a result of the transformation of soluble fibrinogen into insoluble fibrin with the help of fibrin stabilization factor XIIIa.[2] Platelet-rich fibrin (PRF) formed a tetramolecular structure containing cytokines, platelets, and stem cells.[2] Simonpieri et al.[3] concluded in their article that clinical sciences need practical solutions; therefore, only inexpensive and efficient techniques like PRF will continue to develop in the future.

Ankaferd blood stopper (ABS) (Ankaferd Health Products Ltd., Istanbul, Turkey) is a standardized herbal extract that has been approved for external bleeding hemostasis. It is a final product of five different plants (Thymus vulgaris, Glycyrrhiza glabra, Vitis vinifera, Alpinia officinarum, and Urtica dioica).[4],[5] Mechanism of the ABS depends on forming an encapsulated protein network as soon as touching blood. This network provides focal attractive points for blood cell aggregation. This mechanism works independently from coagulation factors and platelets.[5]

The aim of this study was to determine how PRF and ABS loaded PRF responds to the forces applied. Comparison of the mechanical characters of these two membranes is aimed.


   Materials And Methods Top


This study was approved by the Local Ethics Committee of Animal Experiments of Kırıkkale University; the decision is dated 12.27.2016 and numbered 16/92.

A total of 25 adult (12 males and 13 females) New Zealand rabbits (12-month-old) weighing an average of 3000 g were used for the study. The experimental animals were housed in appropriate cages maintained at a temperature of 22°C ± 2°C and a 12-h light/dark cycle. They were transferred to the laboratory environment at least a week before surgery to provide adequate health conditions, protect them from infections, help them in adapting to the new environment, and control their general health conditions. The animals were fed with standard laboratory food and water. Each 1 was housed in a separate cage to ensure comfortable access to water and food, an adequate range of motion, and a stress-free environment.

A mold was designed to obtain samples identical in size, volume, and figure. Mold was done with orthodontic bands and acrylic. For easy handling by the tester, the mold was prepared in 20 mm × 5 mm.

Marginal ear vein was used for blood collection. The rabbit was restrained by one of the researchers with the help of piece of cloth. The ear was warmed by gentle stroking actions to dilate the vein for successful collection. Venipuncture area was cleaned with 70% isopropyl alcohol. The ear was stretched away from the animal before needle insertion. In case of failed blood collection repeated insertion was done at a proximal part of the vein. A volume of 5–7 ml blood was drained passively from the winged blood collection needle to the test tube. Bleeding was stopped by applying gentle pressure on venipuncture site with the help of gauze and fingers.

Collected samples were placed as soon as possible in a centrifugal machine at 3000 rpm for 10 min. After this, three layers formed: lower fraction consists of red blood cells, middle fraction fibrin clot, and the upper part acellular plasma. The middle layer was used to form a membrane. Fibrin clot was placed on the grid of the PRF box and gently compressed with the lid of the box and placed there for 10 min to let squeezing out the fluids to standardize the thickness of the membranes. Two similar samples were punched out from the prepared membrane with the help of the mold [Figure 1]. One of the samples placed in 20% ABS 80% Saline solution for 5 min. Finally, one pure PRF membrane and one ABS loaded membrane obtained from the same sample. This procedure was done to minimalize the differences in samples. Procedure repeated for all of the samples.
Figure 1: Two similar samples prepared by the help of the mold

Click here to view


Tensile test

Mechanical properties of the membrane samples were performed using universal (Zwick/Roell Gruppe/Ulm/Germany) with crosshead speed of 50 mm/min and gauge length of 20 mm according to (UK) [Figure 2]. An average of 5 test results has been reported.
Figure 2: Test machine

Click here to view


Electron microscope analysis

Surface morphology of the samples was examined by electron microscopy [Figure 3]. Samples were washed with PBS and fixed with 2.5% glutaraldehyde (in PBS) for 20 min and dehydrated in ethanol solutions of 50%, 70%, 80%, 90%, and 100% for 5 min each. Followed by drying with 100% hexamethyldisilazane (HMDS) (Sigma Aldrich) for 3 min; excess HMDS was removed and samples aerated overnight. They were mounted on stubs and sputter-coated with platinum. Morphology was examined with JEOL LV 5610 SEM operating at an acceleration voltage of 20KV.
Figure 3: (a) The well-organized dense and mature fibrin matrix with a few cellular structures. (b) Ankaferd Hemostat (ankaferd blood stopper)-induced protein aggregates over and inside the fibrin matrix. Connections are seen between ankaferd blood stopper particles and fibrin matrix

Click here to view


Statistical analysis

All statistical analysis was performed using IBM SPSS Statistics for Windows version 23 software (SPSS Inc., Chicago, IL, USA). Shapiro–Wilk test was used to evaluate the normality assumption. Data are presented as the mean ± standard deviation or median (min-max). Paired sample t-test and Wilcoxon signed-ranks test were used when appropriate. A value of P < 0.05 was considered statistically significant.


   Results Top


Electron microscopy scans shows connections between ABS particles and fibrin matrix [Figure 3]. Results showed that there is statistically significant difference between PRF and ABS added PRF in dL and elongation at break values [Figure 4] and [Figure 5]. The difference at stress at break and modulus was not significant [Table 1].
Figure 4: Result of dL control/experiment which is statistically significant (P = 0,024)

Click here to view
Figure 5: Result of strain which is statistically significant (P = 0,007)

Click here to view
Table 1: Mean values of the results

Click here to view



   Discussion Top


There are numerous articles about PRF and ABS for their clinical benefit. The idea about strengthening the fibrin network with ABS was based on the known mechanism of ABS about forming protein network. Both of the materials have great effects on tissue healing. The idea of the additive effect of both of them over tissue healing is the reason of this study.

High thrombin level in platelet-rich plasma (PRP) procedure causes tetramolecular or bilateral fibrin junctions which results in a rigid fibrin network. Low level of thrombin in PRF procedure causes trimolecular or equilateral fibrin branch junctions results in flexible, elastic, and strong membranes compared to PRP membranes.[1]

Khorshidi et al.[6] indicated that protocol used since years as 2700 rpm/12 min gives better-polymerized PRF which means stronger membranes than 3000 rpm/10 min protocol. Products of both procedures can be examined regarding mechanical quality. This study focuses on the advantages of adding ABS to PRF membrane; therefore, most preferred method is used in the study. For further studies, 2770 rpm/12 min can be studied and compared with the previous one.

A study performed on similarly aged rabbit blood to lessen possible differentiation related to age, sex, and systemic conditions.

PRF increases bone and soft tissue regeneration without any inflammatory reactions. Studies showed that using PRF alone or combination with grafts has positive effects on hemostasis, bone healing, and maturation.[2] Toffler et al.[7] indicated that PRF improves early wound closure, maturation for the bone grafts, and periodontal soft tissues. Besides PRF also ABS has positive effects on tissue healing, hemostasis, bone growth, and maturation. Aktas et al.[8] stated that ABS augmented immune reactivity of collagen Type 1, collagen Type 3, α-smooth muscle actin, fibronectin, 2 microglobulin, vascular endothelial growth factor, Cyclooxygenase-2, and mononuclear phagocyte marker during the first 7 days of soft-tissue healing of rat extraction sites. Combining PRF with ABS will improve biological properties besides physical ones. At this point, can the combination causes apoptosis of the cells or degradation of the cytokines should be evaluated. Haznedaroglu et al.'s[9] research is the first article about ABS and formed network. They showed that ABS forms a protein network and encapsulated cells in undamaged form. According to this and similar articles, this combination will show superior performance on the biological field. Research needed for biological behavior of the combination.

Most of the studies focused on mixture of graft materials such as freeze dried bone, β-tricalcium phosphate (β-TCP) allograft with PRF which accelerates wound healing. In addition decreases needed graft volume which helps in improved angiogenesis, revascularization and greater new bone formation.[7],[10],[11] Similar to these studies, Kim et al.[12] stated that PRF-mixed TCP showed more rapid bone healing than the rhBMP-2-coated TCP or the TCP-only control in their rabbit anterior sinus wall defect model. Mixing PRF with graft materials forms a “biological connector,” which attracts stem cell to the center of the graft mixture and provides neo-angiogenesis.[13] Choukroun et al.[10] concluded in their PRF review that although cytokines trapped in PRF are gradually released and able to accelerate the cellular phenomenon, the structure of the fibrin network is the key element of all improved PRF healing processes.

In oral surgery procedures, PRF can be used as a resorbable membrane to prevent migration of soft-tissue cells during bone healing periods.[14] Also has the advantage of covering and stabilizing the graft particles. Gassling et al.[15] informed that PRF membrane is superior than a collagen membrane for using as a scaffold for the proliferation of periosteal cells in vitro. In a study, using PRF membrane and connective tissue graft following gingival recession operation compared and results were similar. PRF group shows more patient comfort and enhanced tissue healing.[16]

Gassling et al.[15] studied the coverage of the lateral sinus wall with collagen membrane and PRF and concluded that they have similar results for bone formation and residual bone substitute. Simonpieri et al.[13] published PRF grafting as a new method for grafting protocol. They augmented resorbed maxillary alveolar ridge and sinus lift with a mixture of freeze-dried bone graft, and PRF soaked in metronidazole. Authors used PRF as a membrane barrier between soft-tissue and hard-tissue and pointed out that PRF acted as fibrin bandages and quick closure of the wound was detected. As a result, they removed sutures on the day of three. PRF can easily be used both for particulate graft stabilization and guided tissue regeneration. There are numerous published successful case reports.[7]

PRF membrane degrades rapidly which is a short time corresponding with bone healing period.[2] Higher mechanical properties for membranes that are using for guided tissue regeneration is desired a feature. Kawase et al.[17] suggested heat compression of PRF membrane, which reduces the porosity and surface area results in delayed degradation up to 4 weeks. This study shows ABS improves physical properties of PRF. Electron microcopy images show new network formations between fibrin matrix and ABS molecules that may reduce porosity similar to Kawase's study. Advanced studies can be planned for searching biological properties like degradation time.

Another area of using PRF is sinus lifting procedures. There are various literature studies that PRF is using as graft material or membrane. Mazor et al.[18] studied using PRF as a sole grafting material at a sinus lift procedure with simultaneous implant placement and concluded that using PRF which is an inexpensive and simple method, resulted with a high volume of natural regenerated bone. Toffler et al.[7] informed that they are using PRF membranes as membrane insurance for a possible undetected perforation during lateral window technique for sinus lifting procedure. Choukroun et al.[10] presented a patient which has a sinus perforation occurred in lateral wall sinus lift technique and treated successfully by PRF membrane in their PRF review reports. They also propose using PRF in Sinus lift procedure with a mixture of the graft material and under the incision line for accelerating tissue healing.[10] Similar to sinus cavity, PRF can be used after enucleation of an odontogenic cyst and promotes physiologic healing phenomenon. Choukroun et al.[10] emphasized that as PRF fibrin matrix is better organized, it can more efficiently direct stem cell harnessing and the healing program. Cystic cavity heals within 2 months instead of 6–12 months which is required physiologically.

The mold was prepared as an idea gotten from the Khorshidi et al.'s[6] article. The mold was prepared in a rectangular shape instead of dog bone shape to make preparation easier to produce and repeat.

Considering these findings, PRF can be named as a natural fibrin-based biomaterial favorable to increasing vascularization and able to guide epithelial cell migration to its surface. The membrane has a significant positive effect on protecting open wounds and accelerating healing. Furthermore, containing leukocytes and promoting their migration seems handy for using infected wounds.[10] Improving the physical properties of the fibrin matrix, like delayed degradation, can help for better clinical results.


   Conclusion Top


The study shows that mechanical properties of ABS loaded PRF causes better physical properties. Increased strength seems to be due to the additional network over fibrin meshes. Solution percentage of ABS can be determined with different concentrations for the best result. Advanced studies can be done on biological properties, especially on tissue healing.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
   References Top

1.
Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, et al. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part I: Technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:e37-44.  Back to cited text no. 1
    
2.
Borie E, Oliví DG, Orsi IA, Garlet K, Weber B, Beltrán V, et al. Platelet-rich fibrin application in dentistry: A literature review. Int J Clin Exp Med 2015;8:7922-9.  Back to cited text no. 2
    
3.
Simonpieri A, Del Corso M, Vervelle A, Jimbo R, Inchingolo F, Sammartino G, et al. Current knowledge and perspectives for the use of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in oral and maxillofacial surgery part 2: Bone graft, implant and reconstructive surgery. Curr Pharm Biotechnol 2012;13:1231-56.  Back to cited text no. 3
    
4.
Ercetin S, Haznedaroglu IC, Kurt M, Onal IK, Aktas A, Kurt OK, et al. Safety and efficacy of ankaferd blood stopper in dental surgery. Int J Hematol Oncol 2010;20:1-5.  Back to cited text no. 4
    
5.
Goker H, Haznedaroglu IC, Ercetin S, Kirazli S, Akman U, Ozturk Y, et al. Haemostatic actions of the folkloric medicinal plant extract ankaferd blood stopper. J Int Med Res 2008;36:163-70.  Back to cited text no. 5
    
6.
Khorshidi H, Raoofi S, Bagheri R, Banihashemi H. Comparison of the mechanical properties of early leukocyte- and platelet-rich fibrin versus PRGF/Endoret membranes. Int J Dent 2016;2016:1849207.  Back to cited text no. 6
    
7.
Toffler M, Toscano N, Holtzclaw D, Corso MD, Ehrenfest DD. Introducing Choukroun's platelet rich fibrin (PRF) to the reconstructive surgery milieu. J Implant Adv Clin Dent 2009;1:21-30.  Back to cited text no. 7
    
8.
Aktas A, Er N, Korkusuz P, Zeybek D, Onur MA, Tan G, et al. Ankaferd induced early soft tissue wound healing in an experimental rat model. Turk Klinikleri J Med Sci 2013;33:10.  Back to cited text no. 8
    
9.
Haznedaroglu BZ, Haznedaroglu IC, Walker SL, Bilgili H, Goker H, Kosar A, et al. Ultrastructural and morphological analyses of the in vitro and in vivo hemostatic effects of ankaferd blood stopper. Clin Appl Thromb Hemost 2010;16:446-53.  Back to cited text no. 9
    
10.
Choukroun J, Diss A, Simonpieri A, Girard MO, Schoeffler C, Dohan SL, et al. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part V: Histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:299-303.  Back to cited text no. 10
    
11.
Yilmaz D, Dogan N, Ozkan A, Sencimen M, Ora BE, Mutlu I, et al. Effect of platelet rich fibrin and beta tricalcium phosphate on bone healing. A histological study in pigs. Acta Cir Bras 2014;29:59-65.  Back to cited text no. 11
    
12.
Kim BJ, Kwon TK, Baek HS, Hwang DS, Kim CH, Chung IK, et al. A comparative study of the effectiveness of sinus bone grafting with recombinant human bone morphogenetic protein 2-coated tricalcium phosphate and platelet-rich fibrin-mixed tricalcium phosphate in rabbits. Oral Surg Oral Med Oral Pathol Oral Radiol 2012;113:583-92.  Back to cited text no. 12
    
13.
Simonpieri A, Del Corso M, Sammartino G, Dohan Ehrenfest DM. The relevance of Choukroun's platelet-rich fibrin and metronidazole during complex maxillary rehabilitations using bone allograft. Part II: Implant surgery, prosthodontics, and survival. Implant Dent 2009;18:220-9.  Back to cited text no. 13
    
14.
Chang YC, Zhao JH. Effects of platelet-rich fibrin on human periodontal ligament fibroblasts and application for periodontal infrabony defects. Aust Dent J 2011;56:365-71.  Back to cited text no. 14
    
15.
Gassling V, Purcz N, Braesen JH, Will M, Gierloff M, Behrens E, et al. Comparison of two different absorbable membranes for the coverage of lateral osteotomy sites in maxillary sinus augmentation: A preliminary study. J Craniomaxillofac Surg 2013;41:76-82.  Back to cited text no. 15
    
16.
Jankovic S, Aleksic Z, Klokkevold P, Lekovic V, Dimitrijevic B, Kenney EB, et al. Use of platelet-rich fibrin membrane following treatment of gingival recession: A randomized clinical trial. Int J Periodontics Restorative Dent 2012;32:e41-50.  Back to cited text no. 16
    
17.
Kawase T, Kamiya M, Kobayashi M, Tanaka T, Okuda K, Wolff LF, et al. The heat-compression technique for the conversion of platelet-rich fibrin preparation to a barrier membrane with a reduced rate of biodegradation. J Biomed Mater Res B Appl Biomater 2015;103:825-31.  Back to cited text no. 17
    
18.
Mazor Z, Horowitz RA, Del Corso M, Prasad HS, Rohrer MD, Dohan Ehrenfest DM, et al. Sinus floor augmentation with simultaneous implant placement using Choukroun's platelet-rich fibrin as the sole grafting material: A radiologic and histologic study at 6 months. J Periodontol 2009;80:2056-64.  Back to cited text no. 18
    


    Figures

  [Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5]
 
 
    Tables

  [Table 1]



 

Top
  
 
  Search
 
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

 
  In this article
    Abstract
   Introduction
    Materials And Me...
   Results
   Discussion
   Conclusion
    References
    Article Figures
    Article Tables

 Article Access Statistics
    Viewed484    
    Printed28    
    Emailed0    
    PDF Downloaded280    
    Comments [Add]    

Recommend this journal