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  Indian J Med Microbiol
 

Figure 4: Representative photomicrographs of cytoplasmic immunoreactivity for activated caspase-3 of all groups. (a) Group 1 (control); (b) negative control group, no immunohistochemical staining is seen in tracheal tissue; (c) Group 2 (5 ppm FA, 4 weeks), mild immunohistochemical staining (+); (d) Group 3 (5 ppm FA + NS, 4 weeks), mild immunohistochemical staining (+); (e) Group 4 (10 ppm FA, 4 weeks), mild immunohistochemical staining (+); (f) Group 5 (10 ppm FA + NS, 4 weeks), mild immunohistochemical staining (+); (g) Group 6 (5 ppm FA, 13 weeks), mild immunohistochemical staining (+); (h) Group 7 (5 ppm FA + NS, 13 weeks), mild immunohistochemical staining (+); (i) Group 8 (10 ppm FA, 13 weeks), ciliated cells and basal cells of the respiratory epithelium is strongly immunohistochemical staining for active caspase 3; (j) Group 9 (10 ppm FA + NS, 13 weeks), after the NS treatment it is seen that the reduction in the number of immunopositive cells. FA = Formaldehyde; NS = Nigella sativ

Figure 4: Representative photomicrographs of cytoplasmic immunoreactivity for activated caspase-3 of all groups. (a) Group 1 (control); (b) negative control group, no immunohistochemical staining is seen in tracheal tissue; (c) Group 2 (5 ppm FA, 4 weeks), mild immunohistochemical staining (+); (d) Group 3 (5 ppm FA + NS, 4 weeks), mild immunohistochemical staining (+); (e) Group 4 (10 ppm FA, 4 weeks), mild immunohistochemical staining (+); (f) Group 5 (10 ppm FA + NS, 4 weeks), mild immunohistochemical staining (+); (g) Group 6 (5 ppm FA, 13 weeks), mild immunohistochemical staining (+); (h) Group 7 (5 ppm FA + NS, 13 weeks), mild immunohistochemical staining (+); (i) Group 8 (10 ppm FA, 13 weeks), ciliated cells and basal cells of the respiratory epithelium is strongly immunohistochemical staining for active caspase 3; (j) Group 9 (10 ppm FA + NS, 13 weeks), after the NS treatment it is seen that the reduction in the number of immunopositive cells. FA = Formaldehyde; NS = <i>Nigella sativ</i>